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Updated: Dec 16, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
Published on: December 2, 2009
In vivo mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly
Han Wu1, Wen-Jing Zhou1, Lan Liu1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
Abstract:
Herein, we develop a novel tripartite DNA probe to transport phosphorothioated substrate hairpins and an aptamer for the intramolecular CHA circuit, which achieves detection of a low amount of specific mRNA in living cells and mice. Our study provides an improved strategy to promote in vivo fluorescence imaging applications in early-stage clinical diagnosis.
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