Novel electrochemiluminescence solid-state pH sensor based on an i-motif forming sequence and rolling circle...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Materials Science

Background:

• Accurate pH monitoring is crucial in biological and chemical systems.
• Existing pH sensors face limitations in stability and applicability.
• Developing novel sensing platforms for physiological pH is an ongoing challenge.

Purpose of the Study:

• To develop a novel electrochemiluminescence (ECL) solid-state pH sensor.
• To utilize a pH-dependent i-motif forming sequence and rolling circle amplification (RCA) for sensing.
• To demonstrate the sensor's applicability in determining glucose concentrations.

Main Methods:

• Design of a pH-dependent i-motif forming DNA sequence.
• Integration of the i-motif sequence with rolling circle amplification (RCA).
• Fabrication of an electrochemiluminescence (ECL) based solid-state sensor platform.

Main Results:

• The developed sensor exhibited a wide dynamic response range for pH detection, from pH 4 to 7.4.
• The sensor demonstrated successful application in quantifying glucose levels.
• The solid-state nature of the sensor enhances its stability and potential for practical use.

Conclusions:

• A novel ECL solid-state pH sensor based on i-motif and RCA was successfully developed.
• The sensor offers a reliable method for monitoring physiological pH.
• The sensor's ability to detect glucose highlights its versatility and practical biomedical applications.