A comparison of the effects of three androgens on sexual differentiation in female hamsters

The effectiveness of the synthetic androgen 17 beta-hydroxy-17 alpha-methyl-estra-4,9,11-triene-3-one (R 1881), 5 alpha-dihydrotestosterone (DHT), and testosterone to suppress the development of lordotic behavior in female hamsters were compared. Selection of these three androgens was based upon their ability to identify the active agent in defeminization. While all three hormones bind with high affinity to CNS androgen receptors, R 1881 differs from DHT because it is presumably not metabolized into less potent androgens and differs from testosterone because it is presumably not metabolized into estrogen. At birth, female hamsters were given either a single injection of 100 micrograms of hormone, five daily injections of 100 micrograms of hormone, or implanted with Silicone elastomer capsules containing hormone. Controls consisted of hamsters receiving oil injections or cholesterol implants. As adults, the hamsters wee gonadectomized, injected with estradiol benzoate and progesterone and then tested for lordosis. A single injection of androgen at birth was ineffective in suppressing lordosis duration in female hamsters. Multiple injections and implants of R 1881 or testosterone inhibited the development of female sexual behavior. R 1881 administered as five daily injections or implanted for seven days caused a similar partial reduction in lordosis duration. Testosterone was more effective in inhibiting receptivity when given as implants rather than injection. No differences were observed between females receiving testosterone implants at birth and males. DHT had no appreciable effect upon the development of behavior regardless of the route of administration or the length of treatment.(ABSTRACT TRUNCATED AT 250 WORDS)