Related Experiment Video
Updated: Dec 16, 2025
07:49
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
Published on: May 30, 2025
2.1K
Advances of epigenetic editing.
Rutger A F Gjaltema1, Marianne G Rots2
1Otto-Warburg-Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany.
Current Opinion in Chemical Biology
|July 4, 2020
Summary
Epigenetic editing uses CRISPR-dCas9 technology to precisely alter gene expression. This review highlights its applications in mammals and biochemical advancements for versatile therapeutic uses.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Epigenetic editing precisely targets genomic sites to modify the local epigenetic landscape.
- CRISPR-dCas9 systems have significantly advanced epigenetic editing technologies.
- These tools have shown promise in preclinical therapeutic applications.
Purpose of the Study:
- To review current applications of epigenetic editing tools in mammals.
- To discuss biochemical improvements enhancing the versatility of these tools.
- To provide insights into future directions for epigenetic editing.
Main Methods:
- Review of existing literature on epigenetic editing.
- Analysis of CRISPR-dCas9 based systems for locus-specific epigenetic modification.
- Examination of various epigenetic enzymes used in editing strategies.
Main Results:
- Epigenetic editing tools, particularly CRISPR-dCas9, are widely applied in mammalian systems.
- Significant preclinical therapeutic successes have been achieved.
- Biochemical refinements are expanding the scope and precision of these tools.
Conclusions:
- Epigenetic editing offers a powerful approach for transcriptional reprogramming.
- Ongoing advancements in biochemical strategies are crucial for broadening therapeutic potential.
- Further research will likely uncover novel applications in disease treatment and biological research.
Keywords:
CRISPR/dCasEpigenome editingExpression reprogrammingTargeted DNA methylationTargeted histone modificationsMore Related Videos
10:44In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
Published on: May 5, 2023
1.7K
13:47Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
Published on: March 29, 2019
10.1K
Related Concept Videos
01:37Epigenetic Regulation
3.6K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.6K
01:46Epigenetic Regulation
33.2K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.2K
01:59CRISPR
56.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
56.9K
02:23RNA Editing
9.6K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.6K
01:28CRISPR/Cas9 Genome Editing
1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
01:32Chromatin Modification in iPS Cells
2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K