Jove
Visualize
Contact Us

Related Concept Videos

CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
Epigenetic Regulation01:46

Epigenetic Regulation

33.4K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.4K
Epigenetic Regulation01:37

Epigenetic Regulation

3.7K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.7K
RNA Editing02:23

RNA Editing

9.7K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.6K
Overview
79.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same author

Neuron-specific epigenetic repression of <i>Cdk5</i> impairs hippocampal-dependent memory in male and female mice.

bioRxiv : the preprint server for biology·2026
Same author

Hippocampal estrogen levels, receptor types, and epigenetics contribute to sex-specific memory vulnerabilities to concurrent acute stresses.

Neuron·2026
Same author

Structural probing of RNA hairpins quantifies protein occupancy on RNA and links it to function in human cells.

bioRxiv : the preprint server for biology·2026
Same author

Hippocampal Cdk5 is regulated by distinct stress paradigms in male and female mice.

bioRxiv : the preprint server for biology·2026
Same author

Bifunctional transcriptional effector domains control gene expression pulses in an occupancy-dependent manner.

bioRxiv : the preprint server for biology·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 10, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.2K

Epigenetic editing: from concept to clinic.

Elizabeth A Heller1, Lacramioara Bintu2, Marianne G Rots3

  • 1Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USA.

Nature Reviews. Drug Discovery
|November 25, 2025
PubMed
Summary
This summary is machine-generated.

Epigenetic editing offers a novel therapeutic approach by reprogramming gene expression without altering the genome. Despite early challenges, ongoing research is enhancing its efficacy and specificity for treating diseases.

More Related Videos

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.3K
Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

359

Related Experiment Videos

Last Updated: Jan 10, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.2K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.3K
Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

359

Area of Science:

  • Biochemistry
  • Genetics
  • Molecular Biology

Background:

  • Dysregulated gene expression underlies most human diseases.
  • Epigenetic modifications are key mediators of gene expression.
  • Epigenetic editing offers a way to modulate gene expression without genomic alteration.

Purpose of the Study:

  • To review the progress and challenges in epigenetic editing.
  • To highlight the therapeutic potential of epigenetic editing.
  • To discuss future directions for advancing epigenetic editing technologies.

Main Methods:

  • Review of existing literature on epigenetic editing.
  • Analysis of successes in animal models and early clinical trials.
  • Discussion of mechanistic understanding and technological advancements.

Main Results:

  • Epigenetic editing has shown promise in animal models for various diseases.
  • The first clinical trials for epigenetic editing have commenced.
  • Increased understanding of epigenetic reprogramming is addressing initial efficacy and specificity concerns.

Conclusions:

  • Epigenetic editing is evolving into a potent therapeutic strategy.
  • Overcoming challenges in specificity, maintenance, and delivery is crucial for widespread application.
  • Future advancements will solidify epigenetic editing as a powerful therapeutic approach.