Facial rejuvenation using stem cell conditioned media combined with skin needling: A split-face comparative study

Area of Science:

• Regenerative Medicine
• Dermatology
• Aesthetics

Background:

• Stem cell-derived growth factors offer novel facial rejuvenation treatments.
• Skin needling is a popular method for addressing skin aging.
• Combining these modalities may enhance therapeutic efficacy for skin aging.

Purpose of the Study:

• To compare the efficacy of amniotic fluid mesenchymal stem cell derived conditioned media (AF-MSC-CM) plus skin needling against skin needling alone for facial aging.
• To evaluate the combined treatment's impact on managing and delaying facial aging signs.

Main Methods:

• Ten volunteers with facial aging underwent five skin needling sessions bi-weekly.
• AF-MSC-CM was topically applied to the right side post-needling; the left side received needling only.
• Clinical, histological, and morphometrical assessments were performed one month after the final session.

Main Results:

• The combination of AF-MSC-CM and dermaroller (DR) showed a statistically significant improvement in aged skin compared to DR alone (P < .001).
• Dermal structure remodeling was predominantly observed on the side treated with the combined therapy.
• Histometry confirmed a significant increase in epidermal thickness on both treated sides.

Conclusions:

• AF-MSC-CM combined with skin needling is more effective for managing facial aging than skin needling alone.
• This combination therapy presents a promising strategy for advanced skin rejuvenation and anti-aging treatments.