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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Sensors for disposable bioreactors.

Christoph Busse1, Philipp Biechele1, Ingo de Vries1

  • 1Institute of Technical Chemistry Leibniz University Hannover Germany.

Engineering in Life Sciences
|July 7, 2020
PubMed
Summary

New sensors are crucial for monitoring single-use bioreactors, focusing on cost-effective, non-invasive technologies. Addressing interface standardization is key for advancing bioprocess control.

Keywords:
Bioprocess monitoringDisposable bioreactorsDisposable sensorsProcess analytical technologyProcess controlSensor systemsSingle‐use

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Sensor Technology

Background:

  • Single-use bioreactors are increasingly vital in biotechnological applications.
  • Traditional sensors for reusable bioreactors have different requirements than those for disposable systems.
  • Cost-effectiveness and suitability for disposable use are key for sensors in single-use bioreactors.

Purpose of the Study:

  • To provide an overview of current and emerging sensor technologies for single-use bioreactors.
  • To categorize sensors based on their interface with the bioreactor system.
  • To highlight the need for standardized interfaces in single-use bioprocess monitoring.

Main Methods:

  • Review of non-invasive, in-situ sensors utilizing electromagnetic, semiconducting, optical, and ultrasonic principles.
  • Inclusion of emerging technologies such as radio-frequency identification sensors and free-floating sensor spheres.
  • Organization of sensor types by their interface to the bioreactor.

Main Results:

  • Identified various sensor types suitable for single-use bioreactors, emphasizing non-invasive and in-situ approaches.
  • Highlighted emerging technologies like RFID sensors and sensor spheres.
  • Confirmed the current lack of a standard interface for sensors in single-use bioreactors.

Conclusions:

  • Sensor requirements for single-use bioreactors differ significantly from reusable systems, prioritizing cost and disposability.
  • Non-invasive and in-situ sensor technologies are central to advancing single-use bioprocess monitoring.
  • Standardization of sensor interfaces is essential for the future development and widespread adoption of single-use bioprocess monitoring and control.