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Updated: Dec 16, 2025

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Published on: July 19, 2022
Engineering aspects of immobilized lipases on esterification: A special emphasis of crowding, confinement and
Surendra Kumar Parashar1, Sunil Kumar Srivastava1, N N Dutta1
1Department of Chemical Engineering/Chemistry Jaypee University of Engineering and Technology Guna Madhya Pradesh India.
Abstract:
Cross-linked enzyme crystal (CLEC) and sol-gel entrapped pseudomonas sp. lipase were investigated for the esterification of lauric acid with ethanol by considering the effects of reaction conditions on reaction rate. The activation energy for the reaction was estimated to be 1097.58 J/mol and 181.75 J/mol for sol-gel and CLEC entrapped lipase respectively. CLEC lipase exhibited a marginal internal diffusion effect on reaction rate over sol-gel lipases and found to be interesting. The overall reaction mechanism was found to conform to the Ping Pong Bi Bi mechanism. The higher efficiency of sol-gel lipases over CLEC lipases in esterification reaction is mainly due to the combined effects of crowding, confinement and diffusional limitations.
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