Structural, functional relationship of thymus derived cells of the lizard Calotes versicolor

Structural and functional characteristics of the lymphoid cells of Calotes versicolor were studied using thymocytes and splenocytes isolated on a buoyant density gradient and adherence column. The isolated cells were treated with anti-Calotes thymocytes serum (ATS) and their residual capacity to effect antigen specific migration inhibition and antibody production assessed. Results revealed that antigen specific migration inhibition effector cells could be enriched in less dense nylon wool-adherent fraction and 95% of this fraction were sensitive to ATS. Antigen specific plaque forming cells (PFC) could also be enriched in the same fraction but they were not susceptible to ATS.