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Improved method for multisample evaluation of ligand binding to cells. Application to IL-2 receptor measurement
C Lindqvist1, E L Mogensen, A Koman
1Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Journal of Immunological Methods
|October 26, 1988
Abstract:
A newly developed assay using microtitre plates with removable wells permits rapid measurements of IL-2 receptors. Triplicate or quadruplicate samples of several ligand dilutions are easily handled, thus giving more reliable results. In addition, measurement of binding parameters may be accomplished simultaneously on several cell lines. Results are presented which show that the binding assay may also be used for measuring binding constants of antibodies.