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Updated: Dec 16, 2025

Electrocardiogram Recordings in Anesthetized Mice using Lead II

Electrocardiogram Recordings in Anesthetized Mice using Lead II

Published on: June 20, 2020

Electrocardiogram Recordings in Anesthetized Mice using Lead II.

Tae Woong Ha¹, Bermseok Oh¹, Ji-One Kang²

¹Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University.

Journal of Visualized Experiments : Jove

|July 7, 2020

Summary

A new, simple, and affordable electrocardiogram (ECG) recording method in mice enables sensitive cardiac conduction system evaluation. This cost-effective technique validates autonomic nervous system drug effects, aiding preclinical research.

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Area of Science:

Cardiology
Pharmacology
Animal Models

Background:

The electrocardiogram (ECG) is crucial for assessing cardiac function.
Small animal models, particularly mice, are vital for preclinical research.
In vivo ECG measurements in mice present technical and cost challenges.

Purpose of the Study:

To develop and validate a simple, inexpensive, and efficient in vivo ECG recording method for mice.
To assess the reliability of this method for detecting pharmacological effects on cardiac conduction.

Main Methods:

Anesthesia-based ECG recording in mice.
Administration of autonomic nervous system agonists and antagonists (atropine, carbachol, isoprenaline, propranolol).
Comparison of obtained ECG parameters (heart rate, QTc interval) with previous findings.

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Last Updated: Dec 16, 2025

Electrocardiogram Recordings in Anesthetized Mice using Lead II

Electrocardiogram Recordings in Anesthetized Mice using Lead II

Published on: June 20, 2020

Ambulatory ECG Recording in Mice

Ambulatory ECG Recording in Mice

Published on: May 27, 2010

Programmed Electrical Stimulation in Mice

Programmed Electrical Stimulation in Mice

Published on: May 26, 2010

Main Results:

The protocol successfully detected expected changes in heart rate and QTc intervals following drug administration.
Atropine increased heart rate and QTc; carbachol decreased them.
Isoprenaline increased heart rate and QTc, while propranolol showed no significant effect, aligning with prior research.

Conclusions:

This simplified in vivo ECG method in mice is reliable and sensitive for evaluating cardiac conduction.
It offers a cost-effective screening approach for otherwise technically difficult or expensive ECG measurements.
The protocol supports preclinical drug screening and cardiac research in small animal models.