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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
Revealing Kinetics of Two-Electron Oxygen Reduction Reaction at Single-Molecule Level
Yi Xiao1,2, Jaeyoung Hong3,4, Xiao Wang3,4
1State Key Laboratory of Electroanalytical Chemistry and Jilin Province Key Laboratory of Low Carbon Chemical Power, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, P. R. China.
Abstract:
By combining single-molecule fluorescence microscopy with traditional electrochemical methods, herein we report on the investigation of the electrocatalytic kinetics of two-electron (2e) pathway of oxygen reduction reaction (ORR) on a single Fe3O4 nanoparticle. The kinetic parameters for two-electron ORR process are successfully derived at the single-particle level, and a potential dependence of dynamic heterogeneity among individual nanoparticles is revealed. Furthermore, the performance stability of individual Fe3O4 nanoparticles for 2e ORR process is studied. This study deepens our understanding to the electrocatalytic ORR process, especially the 2e pathway at single-molecule and single-particle levels.
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