Preparation of mononuclear cell subpopulations from normal human peripheral blood

By the use of velocity sedimentation at unit gravity, in combination with methods of cell separation based on differences in density and in surface membrane receptors, it has been possible to obtain concentrates of mononuclear cell subpopulations from normal human peripheral blood. T and non-T lymphocytes have been harvested with a good yield and a high degree of purity. Monocytes may also be efficiently recovered using these procedures.