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Cell Monolayer Translocation Assay.

Elsio A Wunder1

  • 1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA. elsio.wunder@yale.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2020
PubMed
Summary
This summary is machine-generated.

Leptospira translocation across host cells is key to infection. A cell monolayer assay quantifies this ability and assesses cell barrier integrity during pathogen invasion.

Keywords:
Electrical resistanceEukaryotic cellsLeptospiraLeptospiral pathogenesisPolarized monolayersTranslocationTranswell®

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Area of Science:

  • Microbiology
  • Pathogen Biology
  • Cell Biology

Background:

  • Leptospiral virulence relies on host cell translocation.
  • This ability aids immune evasion, dissemination, and infection establishment.
  • Understanding translocation mechanisms is crucial for controlling leptospirosis.

Purpose of the Study:

  • To quantify the translocation competence of Leptospira strains.
  • To evaluate the integrity of eukaryotic cell monolayers during translocation.
  • To provide a method for assessing a key virulence factor.

Main Methods:

  • Utilizing a cell monolayer translocation assay.
  • Quantifying the number of translocated Leptospira.
  • Measuring the integrity of polarized eukaryotic cell monolayers.

Main Results:

  • The cell monolayer translocation assay effectively quantifies Leptospira's ability to cross cell barriers.
  • The assay simultaneously assesses the integrity of the cell monolayer.
  • This method allows for comparative analysis of different Leptospira strains.

Conclusions:

  • Cell monolayer translocation assay is a valuable tool for studying leptospiral virulence.
  • The assay aids in understanding host-pathogen interactions.
  • This research contributes to the development of strategies against leptospirosis.