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Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Washed red cells: theory and practice.

Rebecca Cardigan1, Helen V New2, Hazel Tinegate3

  • 1Department of Haematology, NHS Blood & Transplant, University of Cambridge, Cambridge, UK.

Vox Sanguinis
|July 8, 2020
PubMed
Summary
This summary is machine-generated.

Washing red blood cells removes plasma proteins and reduces potassium, but can affect cell quality and shelf life. Method choice is crucial for optimal outcomes in transfusion medicine.

Keywords:
blood component productionred cell componentstransfusion reactionswashing

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Area of Science:

  • Transfusion Medicine
  • Blood Component Processing

Background:

  • Red blood cell (RBC) washing is employed to mitigate allergic reactions from plasma proteins and manage hyperkalemia risk in transfused patients.
  • Various RBC washing methods exist, with outcomes influenced by RBC age, washing solutions, storage media, and post-wash storage duration.

Purpose of the Study:

  • To evaluate the impact of different red blood cell washing techniques on component quality and storage characteristics.
  • To assess the efficacy of washing in reducing plasma-derived allergens and supernatant potassium levels.

Main Methods:

  • Review of laboratory data and studies examining different RBC washing protocols.
  • Analysis of variables including RBC age, washing solutions, storage mediums, and post-wash storage intervals.
  • Assessment of key quality markers: plasma protein removal, potassium levels, IgA levels, and hemolysis.

Main Results:

  • Washing effectively removes 90-95% of plasma, but IgA reduction is method-dependent.
  • Potassium levels decrease post-washing, but subsequent leakage rates vary by method, impacting shelf life.
  • RBC washing negatively affects quality markers like hemolysis, necessitating a reduced shelf life, particularly after irradiation.

Conclusions:

  • RBC washing is a viable method for reducing plasma proteins and potassium, but careful consideration of the method is essential.
  • The choice of washing technique significantly influences RBC quality, potassium rebound, and overall shelf life.
  • Further research is needed to clarify potential benefits and risks, such as effects on lung or kidney injury, as current data are inconclusive.