Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.4K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.4K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.7K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.7K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.4K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyclodextrin complexes of a globular protein and a lipophilic oligopeptide: the effect of structure and physicochemical properties.

Die Pharmazie·2018
Same author

Effect of cyclodextrins on the degradation rate of benzylpenicillin.

Die Pharmazie·2016
Same author

Soft Drugs VI. The Application of the Inactive Metabolite Approach for Design of Soft β-Blockers1?2.

Pharmaceutical research·2013
Same author

Drug permeation through biomembranes: cyclodextrins and the unstirred water layer.

Die Pharmazie·2012
Same author

Aqueous solubility and true solutions.

Die Pharmazie·2010
Same author

Solubility and permeability of steroids in water in the presence of potassium halides.

Die Pharmazie·2010

Related Experiment Video

Updated: Dec 15, 2025

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
09:22

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives

Published on: February 7, 2017

8.1K

Self-assembly of cyclodextrin complexes: detection, obstacles and benefits.

C Muankaew1, P Saokham2, P Jansook3

  • 1Faculty of Pharmacy, Siam University, Bangkok.

Die Pharmazie
|July 9, 2020
PubMed
Summary

Cyclodextrins (CDs) self-assemble into nanoparticles in water. Understanding their aggregation concentration (cac) is crucial for drug manufacturing and developing novel drug delivery systems.

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.8K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.2K

Related Experiment Videos

Last Updated: Dec 15, 2025

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
09:22

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives

Published on: February 7, 2017

8.1K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.8K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.2K

Area of Science:

  • Pharmaceutical Science
  • Materials Science
  • Supramolecular Chemistry

Background:

  • Cyclodextrins (CDs) are cyclic oligosaccharides widely used as pharmaceutical excipients to form inclusion complexes with lipophilic molecules.
  • Recent observations indicate that CDs and their complexes can self-assemble in aqueous solutions, forming transient clusters, nanoparticles, and microparticles.
  • The formation of these aggregates can impact analytical results and product quality in drug manufacturing.

Purpose of the Study:

  • To investigate the self-assembly behavior of cyclodextrins in aqueous solutions.
  • To determine the critical aggregation concentration (cac) for various cyclodextrins and their complexes.
  • To highlight the implications of cyclodextrin aggregation in pharmaceutical applications and drug delivery.

Main Methods:

  • Determination of critical aggregation concentration (cac) for natural cyclodextrins (αCD, βCD, γCD) and a modified cyclodextrin (HPβCD) in aqueous solutions.
  • Analysis of how guest molecule complexation influences the cac values.
  • Observation and characterization of self-assembled structures like clusters and nanoparticles.

Main Results:

  • The critical aggregation concentration (cac) varies significantly among different cyclodextrins: αCD (~25 mg/ml), βCD (~8 mg/ml), and γCD (~9 mg/ml).
  • HPβCD exhibits a substantially higher cac (~118 mg/ml) in aqueous solutions.
  • The presence of guest molecules can alter the cac of cyclodextrin complexes, either increasing or decreasing it.
  • Transient self-assembled structures (clusters, nanoparticles) are formed by CDs in aqueous solutions.

Conclusions:

  • Cyclodextrins and their complexes can form self-assembled nanoparticles in aqueous solutions, with varying critical aggregation concentrations.
  • The transient nature of these aggregates can lead to challenges in detection and potential issues in pharmaceutical manufacturing.
  • Understanding cyclodextrin aggregation is vital for accurate analytical results, preventing product rejection, and for the development of advanced drug delivery systems.