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Does ICSI for in vitro fertilization cause more aneuploid embryos?
Xiangli Niu1, Jiamin Long1, Fangqiang Gong1
1Research Center for Reproductive Medicine, Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi China.
Molecular Cytogenetics
|July 9, 2020
Summary
Intracytoplasmic sperm injection (ICSI) may lead to a higher rate of embryonic aneuploidy compared to conventional in vitro fertilization (IVF). Oocyte manipulation during ICSI might negatively impact embryo development and increase aneuploidy in human IVF.
Area of Science:
- Reproductive Medicine
- Embryology
- Genetics
Background:
- A significant proportion of human embryos generated via in vitro fertilization (IVF) are aneuploid.
- Factors influencing aneuploidy include maternal age, sperm quality, and oocyte manipulation.
- The impact of insemination method (IVF vs. ICSI) on embryonic aneuploidy remains unclear.
Observation:
- A 47-year-old patient underwent treatment using donated oocytes.
- Oocytes were divided, with half undergoing conventional IVF and the other half ICSI.
- Fertilized oocytes were cultured to blastocyst stage for preimplantation genetic testing for aneuploidies (PGT-A).
Findings:
- Embryos from conventional IVF showed a 11.1% aneuploidy rate (1/9 blastocysts).
- Embryos resulting from ICSI exhibited a 50.0% aneuploidy rate (7/14 blastocysts).
- A euploid blastocyst from conventional IVF resulted in a healthy birth.
Implications:
- ICSI may be associated with a higher incidence of embryonic aneuploidy than conventional IVF.
- In vitro manipulation of oocytes during ICSI could adversely affect human oocytes.
- This finding suggests a potential contributing factor to aneuploid embryo formation in human IVF.
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