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Published on: November 30, 2016
Assessment of Circulating Microribonucleic Acids in Patients With Familial Mediterranean Fever
Ferhat Demİr1, Alper Han Çebİ2, Mukaddes Kalyoncu1
1Department of Pediatric Rheumatology, Karadeniz Technical University Medical Faculty, Trabzon, Turkey.
Objectives:
This study aims to evaluate the plasma expression of microribonucleic acids (miRNAs) that may be associated with the pathogenesis of familial Mediterranean fever (FMF).
Patients And Methods:
Thirty patients with FMF (18 males, 12 females; mean age 9.1±4.7 years; range, 3 to 15.5 years) and 30 age- and sex-matched healthy children (18 males, 12 females; mean age 9.5±4.6 years; range, 4 to 16.5 years) were included in this study. The plasma levels of four candidate miRNAs (miRNA-16, miRNA-155, miRNA-204 and miRNA-451) were measured in all subjects. The plasma levels of miRNAs were analyzed with real- time polymerase chain reaction in attack and remission periods of patients and healthy controls (HCs).
Results:
Plasma miRNA-204 levels of FMF patients were decreased 6.5 fold in remission period compared to HCs (p<0.001). This decrease was more prominent in M694V mutation carriers. Plasma miRNA-155 levels of FMF patients were lower in remission period (p=0.03).
Conclusion:
Our findings showed significant alterations in the plasma expression of miRNA-155 and miRNA-204 in FMF patients compared to HCs. Our data suggest that miRNA-155 and miRNA-204 may be related to the pathogenesis of FMF. Further comprehensive and functional researches may help to clarify the role of miRNAs in FMF and elucidate the pathogenesis of the disease.
Insights
Plasma levels of miRNA-155 and miRNA-204 are altered in familial Mediterranean fever (FMF) patients. These microRNAs (miRNAs) may play a role in FMF pathogenesis, suggesting potential diagnostic or therapeutic targets.
Area of Science:
- Genetics
- Molecular Biology
- Immunology
Background:
- Familial Mediterranean fever (FMF) is a genetic autoinflammatory disorder.
- The pathogenesis of FMF involves complex inflammatory pathways.
- MicroRNAs (miRNAs) are emerging as key regulators in various diseases, including inflammatory conditions.
Purpose of the Study:
- To investigate plasma microRNA (miRNA) expression in children with familial Mediterranean fever (FMF).
- To identify specific miRNAs associated with the pathogenesis of FMF.
- To compare miRNA levels between FMF patients during attack and remission periods and healthy controls.
Main Methods:
- A case-control study involving 30 FMF patients and 30 healthy children.
- Quantitative real-time polymerase chain reaction (PCR) was used to measure plasma levels of four candidate miRNAs (miRNA-16, miRNA-155, miRNA-204, miRNA-451).
- MiRNA levels were analyzed in FMF patients during attack and remission phases, and compared to healthy controls (HCs).
Main Results:
- Plasma miRNA-204 levels were significantly decreased (6.5-fold) in FMF patients during remission compared to HCs (p<0.001).
- This reduction in miRNA-204 was more pronounced in patients carrying the M694V mutation.
- Plasma miRNA-155 levels were also found to be lower in FMF patients during remission (p=0.03).
Conclusions:
- Significant alterations in plasma miRNA-155 and miRNA-204 expression were observed in FMF patients compared to healthy controls.
- These findings suggest that miRNA-155 and miRNA-204 may be implicated in the pathogenesis of FMF.
- Further research is warranted to elucidate the precise role of these miRNAs in FMF and its underlying mechanisms.
