Development of Artificial Plasma Membranes Derived Nanovesicles Suitable for Drugs Encapsulation

Area of Science:

• Biotechnology
• Nanomedicine
• Drug Delivery Systems

Background:

• Extracellular vesicles (EVs) show potential as theranostic tools in various diseases.
• The clinical use of therapeutic nanoparticles drives research into artificial EV design.
• Existing methods for creating functional mimetic nanovesicles can be complex.

Purpose of the Study:

• To present a simplified procedure for generating plasma membrane-derived nanovesicles.
• To demonstrate the efficient encapsulation of therapeutic drugs during nanovesicle assembly.
• To validate the functionality of these artificial EVs for drug delivery and therapeutic effects.

Main Methods:

• Plasma membrane-derived nanovesicles were generated using a simplified procedure.
• Nanoparticle characterization involved Tunable Resistive Pulse Sensing (TRPS), transmission electron microscopy, and flow cytometry.
• Encapsulation of Berberine chloride, Temozolomide, and Givinostat into mimetic EVs was performed.

Main Results:

• The simplified method successfully produced functional nanovesicles capable of drug encapsulation.
• Characterization confirmed the physical and molecular properties of the engineered EVs.
• Delivered drugs (Berberine chloride, Temozolomide, Givinostat) exhibited cytotoxic effects comparable to direct administration.

Conclusions:

• A scalable and straightforward method for generating therapeutic nanovesicles was established.
• These artificial EVs can be modified to carry specific therapeutic payloads for drug delivery.
• The findings support the utility of these engineered nanovesicles in diverse therapeutic contexts.