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Related Experiment Videos

A Core Genome Multilocus Sequence Typing Scheme for Streptococcus mutans.

Shanshan Liu1,2, Xiaoliang Li1, Zhenfei Guo1

  • 1Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Msphere
|July 10, 2020
PubMed
Summary
This summary is machine-generated.

A new core genome multilocus sequence typing (cgMLST) scheme for Streptococcus mutans offers a more reliable method for tracking dental caries pathogens. This approach effectively differentiates closely related strains, improving epidemiological studies of S. mutans.

Keywords:
Streptococcus mutanscgMLSTmolecular biologywhole-genome sequence

Related Experiment Videos

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Streptococcus mutans is a primary cause of dental caries.
  • Epidemiological studies of S. mutans have not widely utilized whole-genome sequencing (WGS)-based core genome multilocus sequence typing (cgMLST).
  • Traditional multilocus sequence typing (MLST) has limitations in differentiating closely related S. mutans isolates.

Purpose of the Study:

  • To develop and validate a novel cgMLST scheme for S. mutans.
  • To assess the discriminatory power and reliability of the new cgMLST scheme compared to MLST.
  • To investigate the population structure and global distribution of S. mutans.

Main Methods:

  • Developed a cgMLST scheme targeting 594 genes from the S. mutans UA159 reference genome.
  • Applied the cgMLST scheme to 80 S. mutans isolates from 40 children and 145 isolates from 10 countries.
  • Compared cgMLST results with traditional MLST.
  • Performed phylogenetic analyses using maximum likelihood and minimum spanning trees.

Main Results:

  • The developed cgMLST scheme identified 68 distinct sequence types (cgSTs), significantly higher than the 35 STs identified by MLST.
  • cgMLST differentiated 82.35% of isolates to a unique sequence type, compared to 11.43% with MLST.
  • Phylogenetic analyses indicated that the cgMLST scheme is more reliable than MLST for S. mutans.
  • The cgMLST scheme revealed a diverse population structure and global distribution of S. mutans.

Conclusions:

  • The new cgMLST scheme provides a robust and highly discriminatory molecular typing method for S. mutans.
  • This cgMLST approach offers superior resolution for epidemiological studies of dental caries pathogens.
  • The scheme has the potential to serve as a benchmark for future global research on S. mutans and dental caries.