Sevoflurane induces neuronal activation and behavioral hyperactivity in young mice
Lei Yang1,2, Hoai Ton2, Ruohe Zhao3
1Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, P. R. China.
Scientific Reports
|July 10, 2020
Summary
Sevoflurane anesthesia increases brain activation and hyperactivity in young mice, unlike other anesthetics. Propofol counteracted these sevoflurane effects, suggesting a novel mechanism for anesthetic-induced agitation.
Area of Science:
- Neuroscience
- Anesthesiology
- Pharmacology
Background:
- Sevoflurane is a widely used anesthetic agent.
- Clinical observations suggest sevoflurane can cause patient agitation.
- The underlying neurobiological mechanisms for this agitation are not well understood.
Purpose of the Study:
- To investigate the effects of sevoflurane on neuronal activation and behavior in young mice.
- To explore the potential mechanisms of sevoflurane-induced agitation.
Main Methods:
- Ten-day-old mice were exposed to sevoflurane, isoflurane, or desflurane.
- Behavioral activity was assessed post-anesthetic administration.
- Neuronal activation was measured using c-Fos expression and calcium imaging.
- Propofol was used to determine the causal link between sevoflurane, neuronal activation, and behavior.
Main Results:
- Sevoflurane significantly increased neuronal activation in the primary somatosensory cortex.
- Sevoflurane induced behavioral hyperactivity in young mice.
- Desflurane did not cause hyperactivity, and isoflurane showed only borderline hyperactivity.
- Propofol attenuated both sevoflurane-induced neuronal activation and hyperactivity.
Conclusions:
- Sevoflurane unexpectedly increases neuronal activation and behavioral hyperactivity in young mice.
- These findings suggest a potential mechanism for sevoflurane-induced agitation.
- Further research is warranted to explore anesthetic-induced hyperactivity via increased neuronal activation.
