Reproducibility of the indirect immunofluorescence test on swollen human spermheads

Sera of 111 vasectomized men and infertile men and women were tested in each of two laboratories with the indirect immunofluorescence test on swollen human spermatozoa to detect antibodies against nuclear antigens specific to sperm. Comparison of the results revealed complete agreement in 110 of the sera. There were seventeen concordant positive sera (eight out of eighteen sera from vasectomized men, eight out of seventeen sera from infertile men, and one out of seventy-six infertile women). Most of the antibodies were of the IgG class.