Rapid, Point-of-Care, Paper-Based Plasmonic Biosensor for Zika Virus Diagnosis

Zika virus (ZIKV) is an increasing global health challenge. There is an urgent need for rapid, low-cost, and accurate diagnostic tests that can be broadly distributed and applied in pandemic regions. Here, an innovative, adaptable, and rapidly deployable bioplasmonic paper-based device (BPD) is demonstrated for the detection of ZIKV infection, via quantification of serum anti-ZIKV-nonstructural protein 1 (NS1) IgG and IgM. BPD is based on ZIKV-NS1 protein as a capture element and gold nanorods as plasmonic nanotransducers. The BPD displays excellent sensitivity and selectivity to both anti-ZIKV-NS1 IgG and IgM in human serum. In addition, excellent stability of BPDs at room and even elevated temperature for one month is achieved by metal-organic framework (MOF)-based biopreservation. MOF-based preservation obviates the need for device refrigeration during transport and storage, thus enabling their use in point-of-care and resource-limited settings for ZIKV surveillance. Furthermore, the versatile design (interchangeable recognition element) of BPDs more generally enables their ready adaptation to diagnose other emerging infectious diseases.