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Circular RNA circDLGAP4 is involved in lung cancer development through modulating microRNA-143/CDK1 axis

Bin Wang1, Peiyan Hua1, Bin Zhao2

  • 1Department of Thoracic Surgery, The Second Hospital of Jilin University , Changchun, Jilin, China.

Cell Cycle (Georgetown, Tex.)
|July 11, 2020
PubMed

Insights

Circular RNA DLGAP4 (circDLGAP4) is elevated in lung cancer, promoting tumor growth. It regulates the microRNA-143/CDK1 pathway, suggesting circDLGAP4 as a potential diagnostic and therapeutic target for lung cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung cancer remains a leading cause of cancer-related mortality worldwide.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including lung cancer.
  • The specific role of circDLGAP4 in lung cancer pathogenesis is not well understood.

Purpose of the Study:

  • To elucidate the function of circDLGAP4 in lung cancer development.
  • To investigate the molecular mechanism underlying circDLGAP4's role in lung cancer.
  • To assess the potential of circDLGAP4 as a biomarker for lung cancer.

Main Methods:

  • Quantitative PCR (qPCR) to detect circDLGAP4 expression in lung cancer tissues and cell lines.
  • In vitro experiments to assess the impact of circDLGAP4 knockdown on lung cancer cell behavior.
  • Analysis of the regulatory relationship between circDLGAP4, microRNA-143 (miR-143), and cyclin-dependent kinase 1 (CDK1).

Main Results:

  • circDLGAP4 expression was significantly upregulated in lung cancer tissues and correlated with advanced TNM stage and metastasis.
  • Suppression of circDLGAP4 inhibited lung cancer cell proliferation, migration, and invasion.
  • circDLGAP4 acts as a sponge for miR-143, leading to increased CDK1 expression, thereby promoting lung cancer progression.

Conclusions:

  • circDLGAP4 plays a crucial role in lung cancer progression by modulating the miR-143/CDK1 axis.
  • circDLGAP4 may serve as a promising biomarker for the diagnosis and a potential therapeutic target for lung cancer treatment.
Keywords:
Lung cancercircDLGAP4cyclin-dependent kinase 1microRNA-143

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