Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunomodulation of in vitro antigen presentation by cations.

K L Smith1, D A Lawrence

  • 1Department of Microbiology and Immunology, Albany Medical College, New York 12208.

Toxicology and Applied Pharmacology
|December 1, 1988
PubMed
Summary

Various metal cations impact T cell activation. Lead (Pb) inhibits antigen presentation without toxicity, suggesting unique biochemical effects on antigen-presenting cells (APCs).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: TRAF2 is a biologically important necroptosis suppressor.

Cell death and differentiation·2025
Same author

Newborn adipokines and early childhood growth.

Pediatric obesity·2018
Same author

Impact of parental obesity on neonatal markers of inflammation and immune response.

International journal of obesity (2005)·2016
Same author

Passenger mutations and aberrant gene expression in congenic tissue plasminogen activator-deficient mouse strains.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Grating coupled SPR microarray analysis of proteins and cells in blood from mice with breast cancer.

The Analyst·2015
Same author

Mechanisms Underlying Astrocyte Endfeet Swelling in Stroke.

Acta neurochirurgica. Supplement·2015

Area of Science:

  • Immunology
  • Cell Biology
  • Toxicology

Background:

  • T cell activation is a complex process involving antigen capture, processing, and presentation by antigen-presenting cells (APCs).
  • Cations can potentially modulate immune responses, but their specific effects on T cell activation pathways require detailed investigation.

Purpose of the Study:

  • To assess the in vitro effects of various cations on antigen-specific T cell activation.
  • To investigate the impact of specific metals on antigen presentation by APCs and T cell hybridoma activation.

Main Methods:

  • An in vitro system was used, analyzing ovalbumin antigen presentation by APCs (splenocytes, B cells, macrophages) and T cell hybridoma DO-11.10 activation.
  • Activation was quantified by interleukin-2 (IL2) production measured via bioassay.

Related Experiment Videos

  • Various metal cations were screened for their modulatory effects on cocultures and individual cellular components.

    • Main Results:

    • Inhibitory effects on T cell activation were observed with Cadmium (Cd) > Copper (Cu) > Lead (Pb) > Zinc (Zn).
    • Nickel (Ni) enhanced IL2 production, while Cobalt (Co) and Chromium (Cr) had no effect.
    • Cd exhibited toxicity to all cell types; Cu was toxic to splenocytes and DO-11.10 cells; Pb inhibited antigen presentation without apparent toxicity.

    Conclusions:

    • Metal cations differentially modulate T cell activation pathways.
    • Lead (Pb) uniquely inhibits antigen presentation via a non-toxic mechanism, warranting further study into its biochemical interactions with APCs.
    • Understanding these cation-specific effects is crucial for comprehending immune system modulation by metals.