Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.4K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.4K
Protein Complex Assembly02:41

Protein Complex Assembly

2.4K
2.4K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

26.7K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
26.7K
Protein Organization01:13

Protein Organization

155.1K
Overview
155.1K
Protein Organization01:24

Protein Organization

8.7K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
8.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.8K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<sup>64</sup>Cu Hypoxia Imaging Radiotracer Targeting the Human Copper Transporter.

Neuromolecular medicine·2026
Same author

Glutamine Tautomerization Drives RhoGAP-Aided GTP Hydrolysis in Small Rho GTPases.

Journal of the American Chemical Society·2026
Same author

Structural flexibility of the human vault particle revealed by high-resolution cryo-EM and molecular dynamics simulations.

Nature communications·2026
Same author

Small RNAs, big potential: Engineering microRNA-based synthetic gene circuits.

Current opinion in chemical biology·2026
Same author

Repurposing nuclear receptors for ligand-responsive liquid condensate formation and gene regulation.

Nature communications·2026
Same author

Self-assembling protein cages: from coiled-coil module to machine learning-driven <i>de novo</i> design of next-generation biomaterials.

Materials advances·2025

Related Experiment Video

Updated: Dec 15, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.3K

Molecular assemblies built with the artificial protein Pizza.

Jeroen P M Vrancken1, Jana Aupič2, Christine Addy3

  • 1Laboratory of Biomolecular Modelling and Design, Department of Chemistry, KU Leuven, Celestijnenlaan 200G, 3001 Leuven, Belgium.

Journal of Structural Biology: X
|July 11, 2020
PubMed
Summary

Researchers engineered self-assembling artificial proteins, creating novel designer nanoparticles. These Pizza-based protein nanoparticles are stable, produced in high yield, and can be functionalized for advanced applications.

Keywords:
BiotechnologyProtein designProtein nanoparticlesStructural biology

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.8K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.5K

Related Experiment Videos

Last Updated: Dec 15, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.3K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.8K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.5K

Area of Science:

  • Protein engineering
  • Biomolecular self-assembly
  • Nanotechnology

Background:

  • Pizza6 and Pizza2 are artificial proteins with propeller folds and self-assembly capabilities.
  • Their symmetrical structure makes them suitable for building larger protein complexes and nanoparticles.

Purpose of the Study:

  • To explore the self-assembly of Pizza2 fused to homo-oligomerizing peptides.
  • To engineer novel, larger symmetrical protein complexes (nanoparticles).
  • To investigate the functionalization of these designer nanoparticles.

Main Methods:

  • Engineering of five different Pizza2-peptide fusion proteins.
  • Characterization of self-assembled complexes using biophysical techniques.
  • Fusion of a successful construct to enhanced green fluorescent protein (eGFP) for functionalization studies.

Main Results:

  • Three out of five engineered fusion proteins successfully assembled into larger complexes.
  • One monodisperse designer protein nanoparticle was obtained with a structure closely matching the design.
  • The Pizza-based nanoparticle was successfully fused with eGFP, demonstrating stability and high yield expression.

Conclusions:

  • Pizza2-peptide fusions can form larger, symmetrical protein nanoparticles.
  • Engineered Pizza-based nanoparticles are stable, producible in high yield, and amenable to functionalization.
  • These designer nanoparticles hold potential for applications in nanotechnology and synthetic biology.