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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
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Updated: Dec 15, 2025

Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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Cleaving DNA by nanozymes.

Ruiqin Fang1, Juewen Liu2

  • 1School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054, China and Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. liujw@uwaterloo.ca.

Journal of Materials Chemistry. B
|July 11, 2020
PubMed
Summary
This summary is machine-generated.

Nanozymes are nanomaterial enzyme mimics with DNA cleavage activity. This perspective highlights recent advancements in nanozyme DNA cleavage, including applications in gene editing and antibacterial agents.

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA cleavage is vital for biological processes like replication and transcription.
  • It is also a key tool in gene editing, therapeutics, and biosensor design.
  • Nanozymes offer stable, cost-effective, and recyclable alternatives to natural enzymes.

Purpose of the Study:

  • To introduce nanozymes with DNA cleavage activities.
  • To focus on recent examples and their cleavage mechanisms.
  • To discuss applications and future research opportunities.

Main Methods:

  • Review of recent literature on nanozymes exhibiting DNA cleavage.
  • Discussion of hydrolytic and oxidative DNA cleavage mechanisms by nanozymes.
  • Exploration of applications in DNA repair and as antibacterial agents.

Main Results:

  • Emergence of nanozymes with significant DNA cleavage activities.
  • Examples include CeO2 nanoparticles, chiral carbon dots, and light-assisted cleavage.
  • Nanozymes demonstrate potential in DNA repair and antibacterial applications.

Conclusions:

  • Nanozymes offer promising avenues for DNA cleavage applications.
  • Further research can expand their utility in biotechnology and medicine.
  • Understanding cleavage mechanisms is key to optimizing nanozyme design.