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Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application.

Célio Jr da Costa Fernandes1, Thaís Silva Pinto1, Ha Ram Kang1

  • 1Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.

Advanced Biosystems
|July 11, 2020
PubMed
Summary

Layered double hydroxides (LDHs) show no cytotoxicity and modulate fibroblast behavior. These nanomaterials impact cell adhesion and migration, offering potential for soft tissue bioengineering applications like wound healing.

Keywords:
bioengineeringbiofabricationdouble metal hydroxidesfibroblasthydrotalcite-like materiallayered double hydroxidesnanomaterials

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Layered double hydroxides (LDHs) are promising nanomaterials for human health applications.
  • Their specific roles in bioengineering, particularly concerning cellular responses, require further investigation.

Purpose of the Study:

  • To evaluate the acute effects of two LDH compositions (Mg2Al-Cl and Zn2Al-Cl) on fibroblasts.
  • To explore the potential of LDHs in soft tissue bioengineering.

Main Methods:

  • Scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDX) to characterize LDH particles.
  • Assessment of cytotoxicity and biocompatibility of LDHs on fibroblasts.
  • Analysis of fibroblast adhesion, actin cytoskeleton reorganization, cell cycle progression, and extracellular matrix remodeling.
  • Functional validation using a wound-healing assay.

Main Results:

  • LDH particles exhibited stability in cell culture medium and adsorbed soluble proteins.
  • LDHs released magnesium, zinc, and aluminum without causing cytotoxic or biocompatibility issues.
  • LDHs interfered with fibroblast adhesion by reorganizing the actin cytoskeleton, preceding cell cycle progression.
  • A wound-healing assay confirmed LDHs' impact on cell migration and extracellular matrix remodeling.

Conclusions:

  • LDHs nanomaterials effectively modulate fibroblast adhesion, proliferation, and migration.
  • These findings represent advancements in biomaterials for soft tissue bioengineering.
  • Further exploration of LDHs is warranted for treating disorders such as burn wound healing.