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Updated: Dec 15, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Fluorescent Bioconjugates for Super-Resolution Optical Nanoscopy.

Zhihe Liu¹, Jie Liu^1,2, Xiaodong Wang¹

¹Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 510855, China.

Bioconjugate Chemistry

|July 11, 2020

Summary

This summary is machine-generated.

Super-resolution imaging breaks the diffraction limit for biological studies using advanced fluorescent probes. This review covers progress in probes like fluorescent proteins and nanoparticles for nanoscale visualization.

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Area of Science:

Biophysics
Nanotechnology
Microscopy

Background:

Fluorescent microscopy is crucial for biological studies but limited by the diffraction limit.
Super-resolution imaging techniques have emerged to overcome these limitations, enabling nanoscale visualization.
Advances in fluorescent probes are intrinsically linked to the development of super-resolution nanoscopy.

Purpose of the Study:

To review recent advancements in fluorescent probe bioconjugates for super-resolution imaging.
To analyze the characteristics and adaptability of various probes in current imaging methods.
To provide a future outlook on developing novel fluorescent probes for enhanced super-resolution imaging.

Main Methods:

Review of existing literature on fluorescent probes and super-resolution imaging techniques.
Categorization and analysis of different types of fluorescent probes, including fluorescent proteins (FPs), organic dyes, quantum dots (Qdots), carbon dots (Cdots), upconversion nanoparticles (UCNPs), aggregation-induced emission (AIE) nanoparticles, and polymer dots (Pdots).
Evaluation of probe properties in relation to their performance in super-resolution nanoscopy.

Main Results:

Significant progress has been made in developing diverse fluorescent probe bioconjugates.
The intrinsic properties of probes critically influence the performance of super-resolution techniques.
Various probes, including FPs, Qdots, Cdots, UCNPs, AIE nanoparticles, and Pdots, have been successfully applied.

Conclusions:

Fluorescent probes are essential for achieving high-performance super-resolution imaging.
Understanding probe characteristics is key to optimizing imaging resolution and applications.
Further development of fluorescent probes is needed to push the boundaries of nanoscale biological visualization.