Larkinella punicea sp. nov., isolated from manganese mine soil

Strain ZZJ9^T is a Gram-stain-negative, rod-shaped, aerobic bacterium isolated from manganese mine soil. Strain ZZJ9^T showed the highest 16S rRNA gene sequence similarities with Larkinella rosea 15J16-1T3A^T (97.1%), Larkinella terrae 15J8-8^T (97.0%), Larkinella knui 15J6-3T6^T (96.8%), and Larkinella ripae 15J11-1^T (95.3%). The genome size of strain ZZJ9^T was 8.01 Mb and the DNA G+C content was 51.8 mol%. ANI values among strain ZZJ9^T and Larkinella rosea 52004 ^T, Larkinella knui KCTC 42998^T, and Larkinella terrae 52001^T were 80.5%, 82.7%, and 80.5%, respectively. dDDH values among strain ZZJ9^T and Larkinella rosea 52004^T, Larkinella knui KCTC 42998^T, and Larkinella terrae 52001^T were 23.5%, 26.0%, and 23.6%, respectively. Furthermore, the genome of strain ZZJ9^T contained 6302 predicted protein-coding genes and 3114 (49%) of them had classificatory functions. The major quinone of strain ZZJ9^T was menaquinone-7 and the main cellular fatty acids were C_16:1ω5c (39.5%), iso-C_15:0 (25.6%), and iso-C_17:0 3OH (11.5%). The polar lipids of strain ZZJ9^T were phosphatidylethanolamine, unidentified lipid, and two unidentified aminolipids. Based on the results of phylogenetic, genome, phenotypic, and chemotaxonomic analytical, strain ZZJ9^T represents a novel species of the genus Larkinella, for which the name Larkinella punicea sp. Nov. is proposed. The type strain is ZZJ9^T (= KCTC 62876^T = CCTCC AB 2018215^T).