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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Multi-species Conserved Sequences02:51

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Next-Generation Sequencing in Equine Genomics.

Jessica L Petersen1, Stephen J Coleman2

  • 1Department of Animal Science, University of Nebraska-Lincoln, ANSC A218g, 3940 Fair Street, Lincoln, NE 68583-0908, USA.

The Veterinary Clinics of North America. Equine Practice
|July 14, 2020
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) has revolutionized equine genomics, enabling researchers to explore genetic variations linked to horse health and performance. This technology translates discoveries into practical clinical applications for equine well-being.

Keywords:
EquineGenetic variationGenomicsRNA sequencingTranscriptome

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Area of Science:

  • Equine genomics
  • Molecular biology
  • Veterinary science

Background:

  • The horse reference genome has been crucial for equine health and performance research.
  • Next-generation sequencing (NGS) represents a significant advancement in equine genomics.

Purpose of the Study:

  • To discuss the history and development of NGS in equine genomics.
  • To detail available NGS platforms and their applications.
  • To explore the use of NGS in both discovery and clinical settings for horses.

Main Methods:

  • Alignment and comparison of DNA and RNA sequencing data to the horse reference genome.
  • Utilizing NGS technologies for exploring genetic variations.
  • Reviewing current NGS platforms and their functionalities.

Main Results:

  • NGS facilitates the exploration of genetic variations associated with equine diseases.
  • NGS enables the translation of research findings into clinical applications for horses.
  • Various NGS platforms offer diverse capabilities for equine genomic studies.

Conclusions:

  • NGS is a transformative technology in equine genomics, impacting health and performance research.
  • The application of NGS ranges from fundamental discovery to direct clinical use in veterinary medicine.
  • Continued development and application of NGS promise further advancements in understanding and managing equine health.