Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD4, CD8 and NK subsets in B-CLL.

F Vuillier1, P Tortevoye, J L Binet

  • 1Unité d'Immuno-Hématologie et d'Immuno-Pathologie, Institut Pasteur, Paris, France.

Nouvelle Revue Francaise D'Hematologie
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unveiling autonomic failure in synucleinopathies: Significance in diagnosis and treatment.

Revue neurologique·2024
Same author

Unusual clinical and topographical midbrain stroke revealed by pure sensory disturbance.

Revue neurologique·2021
Same author

Social and emotional cognition in patients with severe migraine consulting in a tertiary headache center: A preliminary study.

Revue neurologique·2021
Same author

Monoclonal and/or Oligoclonal Immunoglobulins in Sera of Patients with Non-Hodgkin's Lymphomas, Determined by Iso-Electric Focusing and Immunoblotting.

Leukemia & lymphoma·2016
Same author

B Cell Malignancies Frequently Target the Autoreactive B Cell Repertoire.

Leukemia & lymphoma·2016
Same author

Proposals for a Phenotypic Classification of B-Chronic Lymphocytic Leukemia, Relationship with Prognostic Factors.

Leukemia & lymphoma·2016
Same journal

XIVth Congress of the French Society of Hematology. Paris, France, February 3-4, 1995. Abstracts.

Nouvelle revue francaise d'hematologie·1995
Same journal

Improved detection of CD2 on formaldehyde-fixed non dehydrated cells.

Nouvelle revue francaise d'hematologie·1995
Same journal

Engraftment of CD34+ peripheral blood progenitor cells into multiple myeloma patients following total body irradiation.

Nouvelle revue francaise d'hematologie·1995
Same journal

Allogeneic transplantation of peripheral blood progenitor cells.

Nouvelle revue francaise d'hematologie·1995
Same journal

Selection and expansion of CD34+ cells.

Nouvelle revue francaise d'hematologie·1995
Same journal

Ex vivo hematopoietic cell expansion: a theoretical and technological challenge.

Nouvelle revue francaise d'hematologie·1995
See all related articles

In B-cell chronic lymphocytic leukemia (B-CLL), T-cell numbers increase, with specific subsets like helper inducer T-cells and cytotoxic T-lymphocytes showing significant augmentation, indicating a complex immune response.

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • B-cell chronic lymphocytic leukemia (B-CLL) is characterized by malignant B-cell proliferation.
  • Elevated T-cell counts are common in B-CLL, but their specific subsets and roles remain incompletely understood.
  • T lymphocytes are generally considered polyclonal and not derived from the malignant clone in B-CLL.

Purpose of the Study:

  • To precisely define and quantify CD4, CD8, and NK cell subpopulations in B-CLL patients.
  • To investigate the changes in these T-cell subsets in relation to disease stage.
  • To assess T-cell subset recovery during clonal remission.

Main Methods:

  • Utilized double labeling techniques to differentiate CD4 subpopulations into helper inducer (CD4+CDW29+) and suppressor inducer (CD4+CD45R+).

Related Experiment Videos

  • Defined CD8 subpopulations (suppressors, NK cells, CTLs) based on CD8 density and reactivity with CD16 and CD11b.
  • Analyzed T-cell and NK cell subpopulations in 27 B-CLL patients and 47 healthy controls.

    • Main Results:

    • Absolute counts of CD3, CD4, CD8, and NK cells were increased in B-CLL despite decreased percentages.
    • CD8 subpopulations increased more than CD4 subpopulations, indicating an imbalance.
    • Within CD4 cells, helper inducers (CD4+CDW29+) increased, while suppressor inducers (CD4+CD45R+) decreased in later stages.
    • All three identified CD8 subpopulations (NK, suppressors, CTLs) showed increases.
    • In three cases of clonal remission, T-cell and NK subpopulations normalized.

    Conclusions:

    • B-CLL is associated with significant quantitative and qualitative alterations in T-cell and NK cell populations.
    • The observed T-cell subset changes suggest a complex immune dysregulation in B-CLL.
    • T-cell subset recovery during remission indicates potential for immune reconstitution.