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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Published on: May 29, 2014
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Novel Approaches to Profile Functional Long Noncoding RNAs Associated with Stem Cell Pluripotency
11Stem Cell and Cancer Center, The First Hospital of Jilin University, Changchun, Jilin130021, China; 2Division of Endocrinology and Metabolism, The First Hospital of Jilin University, Changchun, Jilin130021, China; 3Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin130021, China.
Current Genomics
|July 14, 2020
Summary
Long noncoding RNAs (lncRNAs) are crucial for stem cell pluripotency and reprogramming. This review highlights methods for profiling these functional lncRNAs in cell differentiation and reprogramming.
Area of Science:
- Stem Cell Biology
- Epigenetics
- RNA Biology
Background:
- Stem cell pluripotency relies on complex gene networks.
- Long noncoding RNAs (lncRNAs) are key regulators without protein-coding functions.
- lncRNAs show dynamic changes during somatic cell reprogramming.
Purpose of the Study:
- To review recent advancements in profiling functional lncRNAs.
- To explore the role of lncRNAs in somatic cell reprogramming and differentiation.
Main Methods:
- Single-cell sequencing identifies dynamic lncRNA expression.
- lncRNAs interact with proteins and chromatin modifiers.
- Profiling methods for functional lncRNA analysis are discussed.
Main Results:
- Hundreds of lncRNAs exhibit dynamic changes during reprogramming.
- lncRNAs are implicated in initiating reprogramming and maintaining pluripotency.
- lncRNAs form intricate networks associated with pluripotency.
Conclusions:
- lncRNAs play significant roles in stem cell pluripotency and reprogramming.
- Advanced profiling techniques are essential for understanding lncRNA function.
- Further research on lncRNAs will illuminate cell differentiation pathways.
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