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Updated: Dec 15, 2025

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Published on: June 9, 2020
A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii
Chihiro Kadooka1,2, Masaaki Yamaguchi2, Kayu Okutsu2
1United Graduate School of Agricultural Sciences, Kagoshima University , Kagoshima, Japan.
Abstract:
We developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1-based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kawachii. The plasmids were easily lost when selective pressure was removed, allowing for successive rounds of genome editing.
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