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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Dec 15, 2025

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method.

Sema Elif Eski¹, Christine Dubois², Sumeet Pal Singh³

¹IRIBHM, Université Libre de Bruxelles (ULB).

Journal of Visualized Experiments : Jove

|July 14, 2020

Summary

This study introduces a rapid, enzyme-free method for isolating nuclei, crucial for high-throughput sequencing. This technique enhances sample preparation robustness for single-cell RNA-seq and ATAC-seq experiments.

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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

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Last Updated: Dec 15, 2025

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

Area of Science:

Molecular Biology
Genomics
Neuroscience

Background:

High-throughput transcriptome and epigenome profiling demand intact single-cell or single-nuclei suspensions.
Current methods involving dissociation and permeabilization can introduce noise, damage, and are challenging for certain cell types like neurons.
Optimization of nuclei isolation protocols is time-consuming, labor-intensive, and costly.

Purpose of the Study:

To develop a rapid, robust, and accessible nuclei isolation method for high-throughput sequencing.
To overcome limitations of existing dissociation and permeabilization techniques.
To improve sample preparation for single-nuclei RNA-seq and ATAC-seq.

Main Methods:

A novel enzyme and detergent-free, column-based nuclei isolation protocol.
Application to the entire zebrafish brain, yielding nuclei within 20 minutes.
Utilized flow cytometry for nuclei enrichment and debris clearance.

Main Results:

Efficient isolation of nuclei with intact morphology and low aggregation.
Rapid protocol completion (under 20 minutes) from zebrafish brain.
Demonstrated suitability for downstream applications like single-nuclei RNA-seq and ATAC-seq.

Conclusions:

The developed protocol offers a simple, rapid, and reproducible method for nuclei isolation.
This technique enhances the robustness of sample preparation for high-throughput genomic studies.
The method is applicable to soft tissues and cultured cells, simplifying single-nuclei sequencing experiments.