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Alpha-naphthyl-acetate-esterase activity in cerebrospinal fluid cells
1Department of Neurology, University Hospital, Gent, Belgium.
Acta Neurologica Scandinavica
|September 1, 1988
Summary
Alpha-naphthyl-acetate-esterase (ANAE) staining of cerebrospinal fluid (CSF) cells can help diagnose neurological diseases. ANAE activity in lymphocytes and mono-histiocytes differs across conditions, aiding in diagnosis and monitoring treatment effectiveness.
Area of Science:
- Neurology
- Cytochemistry
- Immunology
Background:
- Cerebrospinal fluid (CSF) analysis is crucial for diagnosing neurological disorders.
- Cytochemical stains provide valuable information about cellular components in CSF.
Purpose of the Study:
- To evaluate the utility of alpha-naphthyl-acetate-esterase (ANAE) staining in CSF cytology for various neurological diseases.
- To assess changes in ANAE activity in lymphocytes and mono-histiocytes in different neurological conditions.
Main Methods:
- CSF samples from 50 patients with neurological diseases were analyzed.
- Cytochemical staining for alpha-naphthyl-acetate-esterase (ANAE) was performed on mono-histiocytes and T-lymphocytes.
Main Results:
- Decreased ANAE activity in lymphocytes was observed in multiple sclerosis and subacute sclerosing panencephalitis.
- Increased ANAE activity in mono-histiocytes was noted in CNS infarctions and bacterial/viral infections.
- An increase in ANAE-positive lymphocytes correlated with treatment and improvement in bacterial meningitis and viral meningo-encephalo-radiculitis.
Conclusions:
- ANAE staining is a potentially useful adjunct in CSF cytology for diagnosing and monitoring neurological conditions.
- Differential ANAE activity in CSF cells can help distinguish between various neurological diseases.