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An immunogold cell labelling method for rat T lymphocytes
G O Evans1, R M Flynn, J D Lupton
1Department of Toxicology and Metabolism, Wellcome Research Laboratories, Beckenham, Kent, UK.
Laboratory Animals
|October 1, 1988
Abstract:
A method using monoclonal antibodies in conjunction with an immunogold procedure was adapted for labelling T lymphocytes in blood samples obtained from male Wistar rats. The monoclonal antibodies W3/25, MRC/OX8 and W3/13 were used. Changes in the percentages of positively labelled cells were observed in rats dosed with the immunosuppressant cyclosporin.