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Related Experiment Video

Updated: Dec 15, 2025

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Isotopically Encoded Nanotags for Multiplexed Ion Beam Imaging.

Stefan Harmsen1, Ahmet F Coskun1, Shambavi Ganesh2

  • 1Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Advanced Materials Technologies
|July 15, 2020
PubMed
Summary

A novel nanobarcoding platform enhances multiplexed ion beam imaging (MIBI) by using isotopically encoded nanotags. This technology overcomes mass-channel limitations, enabling more sensitive and high-dimensional single-cell analysis.

Keywords:
barcodesisotopeslabelsmultiplexed ion beam imagingsilica nanoparticles

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Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • High-dimensional single-cell analysis is crucial for understanding biological processes.
  • Existing methods like mass cytometry have limitations in sensitivity and mass-channel capacity.
  • Multiplexed ion beam imaging (MIBI) offers advanced cellular profiling capabilities.

Purpose of the Study:

  • To develop a nanobarcoding platform for multiplexed ion beam imaging (MIBI).
  • To overcome sensitivity and mass-channel limitations in current bioimaging techniques.
  • To enable higher-dimensional profiling of biological samples at the single-cell level.

Main Methods:

  • Fabrication of isotopically encoded nanotags (100 nm) using combinatorial isotope distributions.
  • Development of a four-digit barcoding scheme using deuterium, fluorine, bromine, and iodine isotopes.
  • Implementation of a computational debarcoding method and machine learning for analysis.
  • Application to MIBI using secondary ion beam spectrometry for spatial nanotag distribution quantification.

Main Results:

  • Demonstrated detection of 16 unique nanotag variants (4-digit barcoding).
  • Successfully quantified spatial nanotag distributions in large imaging areas (up to 0.6 mm²).
  • Overcame spectral bounds of mass channels by expanding the labeling palette.
  • Validated the platform as a proof-of-principle for enhanced bioimaging.

Conclusions:

  • Isotopically encoded nanotags significantly boost the performance of MIBI and other elemental bioimaging platforms.
  • The developed nanobarcoding platform offers a sensitive and high-capacity solution for single-cell analysis.
  • This technology advances the field of high-dimensional biological profiling.