Related Experiment Video
Updated: Dec 15, 2025
10:00
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
Published on: October 12, 2015
12.2K
Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing
Gareth W Price1, Joe A Potter1, Bethany M Williams1
1Joseph Banks Laboratories, School of Life Sciences, University of Lincoln, Lincoln, UK.
Methods in Molecular Biology (Clifton, N.J.)
|July 15, 2020
Summary
This study details a method for detecting low concentrations of adenosine triphosphate (ATP) using microelectrode biosensors. This technique aids in understanding cell communication disruptions in diseases like diabetes.
Area of Science:
- Biomedical Engineering
- Cellular Biology
- Analytical Chemistry
Background:
- Cell-to-cell communication is vital for tissue function.
- Purinergic signaling, involving adenosine triphosphate (ATP), is a key mechanism.
- Dysregulated extracellular ATP release is implicated in diseases like diabetes, inflammation, and fibrosis.
Purpose of the Study:
- To describe a step-by-step protocol for utilizing sensitive microelectrode biosensors.
- To enable the detection of low, real-time concentrations of extracellular ATP in vitro.
- To provide a tool for investigating altered cell communication in pathophysiological conditions.
Main Methods:
- Development and application of sensitive microelectrode biosensors.
- Real-time detection of extracellular adenosine triphosphate (ATP) concentrations.
- In vitro experimental setup for monitoring ATP biosensing.
Main Results:
- Demonstration of a reliable method for measuring low, real-time extracellular ATP levels.
- Successful application of microelectrode biosensors for sensitive ATP detection.
- Validation of the biosensing approach for studying cell-to-cell communication.
Conclusions:
- Microelectrode biosensors offer a sensitive platform for real-time ATP detection.
- This method facilitates the study of altered purinergic signaling in disease.
- ATP biosensing represents a potential therapeutic target for inflammatory and fibrotic conditions.
Related Concept Videos
01:45Endocrine Signaling
67.1K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
67.1K
01:27ATP Driven Pumps I: An Overview
9.5K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.5K