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Related Experiment Video

Updated: Dec 15, 2025

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

DASH-type cryptochromes - solved and open questions.

Stephan Kiontke¹, Tanja Göbel¹, Annika Brych¹

¹University of Marburg, Department of Biology, Molecular Plant Physiology and Photobiology, D-35032 Marburg, Germany.

Biological Chemistry

|July 15, 2020

Summary

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DASH-type cryptochromes (cry-DASHs) are versatile flavoproteins. Recent studies reveal their dual roles in DNA repair and photoreception, potentially bridging photolyase and cryptochrome functions across taxa.

Area of Science:

Biochemistry
Molecular Biology
Evolutionary Biology

Background:

Cryptochrome/photolyase family (CPF) proteins are flavoproteins involved in DNA repair or light reception.
DASH-type cryptochromes (cry-DASHs) represent a distinct CPF subclade found across diverse taxa.
Mammalian cryptochromes are crucial for circadian rhythm regulation.

Purpose of the Study:

To investigate the functional roles of DASH-type cryptochromes (cry-DASHs).
To explore the evolutionary relationship between photolyases and cryptochromes.
To understand the dual DNA repair and photoreceptor activities of cry-DASHs.

Main Methods:

Comparative analysis of cry-DASHs across species including Drosophila, Arabidopsis, Synechocystis, and human.

Keywords:

DNA-photolyase DNA-repair cryptochrome photoreceptor protein structure

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Last Updated: Dec 15, 2025

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Circadian Entrainment of Drosophila Melanogaster

Circadian Entrainment of Drosophila Melanogaster

Published on: June 3, 2020

Biochemical assays to assess DNA repair capabilities.

Functional studies to evaluate photoreceptor activity.

Main Results:

Cry-DASHs exhibit DNA repair activity, specifically on single-stranded DNA UV lesions.
Evidence suggests cry-DASHs function as photoreceptors, particularly in microbial organisms.
These findings indicate cry-DASHs may represent transitional forms between photolyases and cryptochromes.

Conclusions:

DASH-type cryptochromes possess both DNA repair and photoreceptor functions.
Cry-DASHs likely evolved from photolyases and may represent an intermediate step towards cryptochromes.
The dual functionality of cry-DASHs highlights their significant role in molecular evolution and biological processes.