Related Experiment Video
Updated: Dec 14, 2025
07:29
Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy ALPPS Procedure
Published on: August 14, 2017
15.2K
Simulated model of RAPID concept: highlighting innate inflammation and liver regeneration
J H Shi1,2, X Yan1, S J Zhang1
1Department of Hepatobiliary and Pancreatic Surgery, Henan Key Laboratory of Digestive Organ Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
BJS Open
|July 16, 2020
Summary
The novel RAPID transplantation technique enhances liver regeneration by promoting IL-6 and M1 macrophage polarization. This simulated model aids in understanding liver regeneration mechanisms for improved surgical strategies.
Area of Science:
- Hepatobiliary surgery
- Transplantation immunology
- Regenerative medicine
Background:
- The resection and partial liver segment II/III transplantation with delayed total hepatectomy (RAPID) concept is a novel approach for non-resectable liver tumors.
- Understanding liver regeneration is crucial for managing 'small for size' liver syndrome in liver resection and transplant surgery.
Purpose of the Study:
- To establish a simulated RAPID model in rats to investigate the mechanisms of liver regeneration.
- To compare liver regeneration and inflammatory markers in RAPID-treated rats versus control hepatectomy groups.
Main Methods:
- A RAPID model was created in rats, involving cold ischemia, reperfusion, portal vein ligation, and staged resection.
- Histology, liver regeneration, and inflammatory markers were analyzed.
- In vitro co-culture systems evaluated the effects of IL-6 and macrophage polarization on hepatocyte viability.
Main Results:
- The RAPID model demonstrated a 100% survival rate, comparable to controls.
- RAPID-treated rats exhibited enhanced liver regeneration with increased IL-6 levels and M1 macrophage polarization.
- In vitro studies showed M1 macrophages promote hepatocyte proliferation via the IL-6/STAT3/ERK pathway.
Conclusions:
- The simulated RAPID model effectively studies liver regeneration profiles.
- IL-6 and M1 macrophage polarization are key mechanisms enhancing liver regeneration in the RAPID model.
- Findings may inform surgical strategies for liver regeneration and patient selection.
