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Updated: Aug 4, 2026
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
A label-free electrochemical DNA biosensor for kanamycin detection based on diblock DNA with poly-cytosine as a high
Xiaoyan He1, Huimin Han1, Wenyu Shi1
1Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P. R. China. hexy09@163.com.
Abstract:
It is urgent to develop a more simple and sensitive method to detect antibiotic residues considering the harm of antibiotic residues in food to the human body. Herein we designed a label-free electrochemical DNA biosensor for the sensitive detection of kanamycin (KAN) based on diblock DNA with a 15-mer of poly-cytosine (poly-C). The diblock DNA can be immobilized on graphene oxide (GO) due to strong physical adsorption between the 15-mer of poly-C and GO. The aptamer of KAN acted as the other block for rapidly binding the target. It can specifically capture the target, which leads to the change of electrochemical signal. Consequently, the DNA biosensor exhibited high sensitivity and specificity towards KAN, the linear range was from 0.05 pM to 100 nM with a detection limit of 0.0476 pM. The developed DNA biosensor was constructed easily and showed promising applications for the detection of antibiotic residues for food safety.
