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Glyceraldehyde 3-Phosphate Dehydrogenase on the Surface of <i>Candida albicans</i> and <i>Nakaseomyces glabratus</i> Cells-A Moonlighting Protein That Binds Human Vitronectin and Plasminogen and Can Adsorb to Pathogenic Fungal Cells via Major Adhesins Als3 and Epa6.

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Moonlighting Proteins at the Candidal Cell Surface.

Dorota Satala1, Justyna Karkowska-Kuleta2, Aleksandra Zelazna1

  • 1Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Gronostajowa 7, 30-387 Krakow, Poland.

Microorganisms
|July 18, 2020
PubMed
Summary
This summary is machine-generated.

Moonlighting proteins on the Candida albicans cell surface are key to fungal virulence and host interaction. Understanding these proteins offers new strategies against candidiasis infections.

Keywords:
Candida yeastadhesioncell wallcomplement systemcontact systemenolaseglyceraldehyde-3-phosphate dehydrogenasemolecular mimicrynon-classical secretionplasminogenprotein moonlightingstress protection

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Area of Science:

  • Mycology
  • Infectious Diseases
  • Molecular Biology

Background:

  • The Candida albicans cell wall is crucial for host interaction and environmental adaptation during infection.
  • Fungal cell surface proteins, both bound and loosely attached, play significant roles in pathogenicity.
  • Loosely attached proteins, particularly 'moonlighting proteins,' exhibit dual intracellular and extracellular functions.

Purpose of the Study:

  • To investigate the role of moonlighting proteins in Candida albicans virulence.
  • To understand the mechanisms by which moonlighting proteins contribute to host colonization and infection.
  • To explore the therapeutic potential of targeting moonlighting proteins in candidiasis.

Main Methods:

  • Analysis of protein localization and function in Candida albicans.
  • Investigation of non-classical secretion pathways for extracellular proteins.
  • Assessment of moonlighting protein involvement in adhesion, host immune response evasion, and stress adaptation.

Main Results:

  • Moonlighting proteins are identified as critical virulence factors in Candida albicans.
  • These proteins mediate adhesion to host cells and interact with host proteolytic systems.
  • Extracellular moonlighting proteins contribute to molecular mimicry and stress resistance, enhancing pathogenicity.

Conclusions:

  • Moonlighting proteins are essential for Candida albicans pathogenicity, contributing to host colonization and immune evasion.
  • Targeting these moonlighting proteins presents a promising avenue for developing novel anti-candidiasis therapies.
  • Further research into moonlighting protein functions can inform new diagnostic and preventive strategies against candidiasis.