Liquid chromatography-ion mobility-high resolution mass spectrometry for analysis of pollutants in indoor dust:...

Dust analysis provides a means to assess the degree of exposure of humans in an indoor environment to various contaminant classes such as flame retardants, pesticides and others. There is increasing interest in non-targeted acquisitions using high resolution mass spectrometry (HRMS) to better capture the contaminant profile. However, these studies are confronted with the challenge of assessing confidence in proposed identifications, particularly when authentic standards are not available. Here, we demonstrate the analysis of dust extracts representing various indoor environments (industrial e-waste processing and domestic) for high-abundance environmental contaminants using a data-independent LC-HRMS approach, incorporating ion mobility spectrometry (IMS) to provide additional characterization capability for the complex samples. Twenty-nine xenobiotic compound identifications were made based on both targeted and non-targeted processing approaches using accurate mass precursor and product ion measurement combined with an ion mobility derived collision-cross section (^TWCCS_N2) determination. Characterization of the repeatability of ^TWCCS_N2 value measurements and their average relative error to compared authentic standards of 0.38% were consistent with various published studies and represent a robust measurement property. ^TWCCS_N2 values were particularly useful in cases where confirmation after the initial dust analysis was performed using a different chromatographic method, due to the gas-phase measurement being unaffected by such changes. Observed compound ^TWCCS_N2 values were then compared to predicted CCS_N2 values obtained using two different machine-learning based predictive techniques. Results from one of the predictive programs indicates a promising avenue for use of these models for supporting compound identification in non-targeted analyses.