A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

Area of Science:

• Biomaterials Science
• Surface Chemistry
• Bioorthogonal Chemistry

Background:

• Mussel adhesive proteins inspire strategies for robust surface adhesion.
• Bioorthogonal chemistry offers precise molecular conjugation methods.
• Developing versatile surface engineering for biomedical implants is crucial.

Purpose of the Study:

• To develop a universal surface engineering strategy using mussel-inspired peptide mimics and bioorthogonal click chemistry.
• To demonstrate the ability to impart various bioactivities to different material surfaces.
• To create functional surfaces for biomedical implants.

Main Methods:

• Synthesized a mussel-inspired peptide mimic, DOPA4-azide, with a bioclickable azide group.
• Utilized the peptide mimic for stable surface adhesion on diverse materials (metallic, inorganic, organic polymers).
• Employed bioorthogonal click chemistry to conjugate dibenzylcyclooctyne- (DBCO-) modified bioactive ligands.

Main Results:

• Achieved stable binding of DOPA4-azide on a wide range of substrates.
• Successfully conjugated various bioactive molecules (antifouling polymers, antibacterial peptides, NO-generating catalysts) via click chemistry.
• Fabricated functional surfaces with antibiofouling, antibacterial, and antithrombogenic properties.

Conclusions:

• The mussel-inspired peptide mimic and bioorthogonal click chemistry strategy provides a versatile platform for surface bioengineering.
• This approach is applicable to diverse substrate materials and biofunctionalities.
• The synergistic combination offers a clean and efficient method for creating advanced biomedical materials.