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Updated: Dec 14, 2025

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Characterizing the Heart Rate Response to the 4 × 4 Interval Exercise Protocol.
Justin J Acala1, Devyn Roche-Willis1, Todd A Astorino1
1Department of Kinesiology, California State University, San Marcos, CA 92096-0001, USA.
High-intensity interval training (HIIT) using the 4x4 protocol shows reduced heart rate (HR) intensity in the first interval. For optimal results, start with supramaximal intensity in the initial interval.
Area of Science:
- Exercise Physiology
- Sports Science
- Cardiovascular Training
Background:
- High-intensity interval training (HIIT) is a popular training method.
- The 4x4 protocol, involving four 4-minute bouts at 85-95% maximal heart rate (HR max), is commonly used.
- Understanding the physiological response to this protocol is crucial for optimizing training.
Purpose of the Study:
- To investigate the heart rate (HR) and power output response during the 4x4 HIIT protocol.
- To determine the time spent within the target HR zone (85-95% HR max) during each interval.
- To analyze the changes in power output across the four intervals.
Main Methods:
- 39 active men and women (VO2 max: 37.0 ± 5.4 mL/kg/min) participated.
- Participants underwent an incremental cycling test to determine VO2 max, HR max, and peak power output (PPO).
- The 4x4 HIIT protocol was performed, with HR and power output continuously monitored.
Main Results:
- Participants spent an average of 12.9 minutes within the 85-95% HR max zone out of 16 total interval minutes.
- Time spent in the target HR zone was significantly lower in the first interval (2.7 min) compared to subsequent intervals (3.4-3.5 min).
- Power output was highest in the first interval (75% PPO) and significantly decreased in intervals 2-4 (54-63% PPO).
Conclusions:
- The initial interval of the 4x4 protocol does not effectively achieve the target 85-95% HR max intensity.
- Power output significantly declines throughout the 4x4 protocol.
- To maximize time within the target HR zone, especially for fitter individuals, initiating the first interval at supramaximal intensity is recommended.
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