Paper-based 1,5-anhydroglucitol quantification using enzyme-based glucose elimination.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensors

Background:

• 1,5-anhydroglucitol (1,5-AG) is a valuable biomarker for glucose level monitoring.
• Conventional methods for 1,5-AG quantification are hindered by glucose interference, necessitating complex sample pretreatment.
• Pyranose oxidase (PROD)-based sensors lack specificity, requiring laborious sample purification.

Purpose of the Study:

• To develop a simplified, one-step paper-based sensor for accurate 1,5-AG detection.
• To overcome the challenge of glucose interference in 1,5-AG quantification.
• To create a cost-effective and user-friendly diagnostic tool.

Main Methods:

• A novel paper-based sensor utilizing glucose oxidase, catalase, and mutarotase was engineered.
• The sensor incorporates two distinct compartments for simultaneous glucose and 1,5-AG quantification.
• The system was validated using water and spiked human urine samples.

Main Results:

• The developed sensor effectively eliminates glucose interference, enabling direct 1,5-AG measurement.
• The sensor demonstrated a limit of detection of 0.9 mg dL-1 for glucose.
• The limit of detection for 1,5-AG was determined to be 3.2 μg mL-1.

Conclusions:

• A one-step paper-based sensor provides a streamlined approach for 1,5-AG quantification.
• This biosensor technology simplifies sample handling and reduces analysis time.
• The sensor holds promise for improved point-of-care diagnostics related to glucose metabolism.