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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Dec 14, 2025

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.

Sylvia A Sarnik¹, Bryan A Sutermaster¹, Eric M Darling^1,2,3,4

¹Center for Biomedical Engineering, Brown University, Providence, Rhode Island, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|July 21, 2020

Summary

This summary is machine-generated.

Mass-added density centrifugation (MADC) is a novel cell sorting technique that enables nonbinary separation of large cell numbers based on surface protein levels. This method offers a faster alternative to existing techniques, improving cell yields for research and therapies.

Keywords:

biomarker cell separation density centrifugation density gradient enrichment high-throughput

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Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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Published on: October 25, 2018

Related Experiment Videos

Last Updated: Dec 14, 2025

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Biotechnology
Cell Biology
Biophysics

Background:

Traditional cell sorting methods like FACS and MACS have limitations in cell yield and separation capabilities.
There is a need for advanced cell sorting techniques that can handle large cell numbers and perform nonbinary separations.

Purpose of the Study:

To develop and validate a novel two-step cell sorting method, mass-added density centrifugation (MADC).
To enable nonbinary separation of large cell populations based on surface protein expression levels.

Main Methods:

MADC involves a "mass-adding" step where microparticles bind to target surface proteins, modulating single-cell density.
Cells are then separated using discontinuous density gradient centrifugation, forming discrete density bands.
Demonstrated utility for both live and fixed cell sorting based on CD44 protein abundance.

Main Results:

MADC successfully separated cells into discrete density bands correlating with CD44 protein levels.
Assays confirmed CD44 quantity in separated cell groups aligned with modulated cell density.
The method proved effective for multiple cell types and both live and fixed cells.

Conclusions:

MADC is a rapid and effective method for nonbinary cell isolation based on surface protein levels.
This technique significantly enhances cell yields, making it advantageous for protein analyses and cell-based therapies.
MADC offers a valuable new tool for basic research and therapeutic applications in cell sorting.