Structural Basis for a Quadratic Relationship between Electronic Absorption and Electronic Paramagnetic Resonance...

Type 1 copper (T1Cu) proteins play important roles in electron transfer in biology, largely due to the unique structure of the T1Cu center, which is reflected by its spectroscopic properties. Previous reports have suggested a correlation between a high ratio of electronic absorbance at ∼450 nm to that at ∼600 nm (R = A₄₅₀/A₆₀₀) and a large copper(II) hyperfine coupling in the z direction (A) in electron paramagnetic resonance (EPR). However, this correlation does not have a clear physical meaning, nor does it hold for many proteins with a perturbed T1Cu center. To address this issue, a new parameter of R' [A₄₅₀/(A₄₅₀ + A₆₀₀)] with a better physical meaning of a fractional S_Cys pseudo-σ to Cu(II) charge transfer transition intensity is defined and a quadratic relationship between R' and A is found on the basis of a comprehensive analysis of ultraviolet-visible absorption, EPR, and structural parameters of T1Cu proteins. We are able to find good correlations between R' and the displacement of copper from the trigonal plane defined by the His₂Cys ligands and the angle between the N_His1-Cu-N_His2 plane and the S_Cys-Cu-axial ligand plane, providing a structural basis for the observed correlation. These findings and analyses provide a new framework for a deeper understanding of the spectroscopic and electronic properties of T1Cu proteins, which may allow better design and applications of this important class of proteins for redox and electron transfer functions.