Evaluation of feeding value of combination of alternate protein sources in White Leghorn layers

1. A study was conducted to evaluate the effects of including graded levels of an alternate protein mixture (APM) containing cottonseed meal, distillery dried grain with solubles and mustard seed meal in a fixed ratio as a substitute for soybean meal in layer diets (hens aged 27 to 46 weeks). The trial studied the possibility of higher inclusion levels of APM with increased dietary concentrations of crude protein (CP) or critical amino acids (CAA). 2. Maize-soybean meal diet was used as the control diet. In experiment 1, APM was included at 0, 120, 160 and 200 g/kg diet (145 g/kg CP). In experiment 2, the control and three test diets (200 g/kg APM) with three levels of CP (145, 155 and 165 g/kg) were used. In experiment 3, a control and four test diets (200 g/kg APM) with four concentrations of CAA (100%, 105%, 110% and 115% relative to the control) were used. In all experiments, each diet was fed ad libitum to nine replicates of 88 birds each, housed in colony cages. 3. In experiment 1, egg production (EP) and feed intake (FI) were not affected by feeding up to 160 g/kg APM, but, at 200 g/kg, EP was significantly reduced. Feed conversion ratio (FCR) for the hens fed the highest level of APM was similar to the control. Egg weight (EW) and egg mass (EM) in the 160 and 200 g/kg AMS groups were lower than the control group. 4. Increase in dietary CP from 145 to 155 g/kg improved EP and reduced FCR to levels similar to the control group. EM in hens fed 165 g/kg APM was similar to the control in experiment 2. 5. Increasing concentrations of CAA progressively improved EP and reduced FCR in experiment 3. The EP at CAA 110% and FCR at CAA 105% were similar to the control group. 6. Based on the results, it was concluded that the APM can be included up to 160 g/kg diet without affecting layer performance, as long as the diet supplied the recommended levels of protein and amino acids. By increasing either protein from 145 to 155 g/kg or CAA concentrations by 10%, the dietary levels of the APM could be increased to 200 g/kg without affecting the layer performance.